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Analytical chemistry articles from across Nature Portfolio
Analytical chemistry is a branch of chemistry that deals with the separation, identification and quantification of chemical compounds. Chemical analyses can be qualitative, as in the identification of the chemical components in a sample, or quantitative, as in the determination of the amount of a certain component in the sample.
And yet it rotates!
Electrocatalysis would not be the same without the rotating disk electrode. Its invention in the mid-twentieth century enabled immense developments, which rendered it a classic technique in electrochemistry. The rotating disk electrode will remain a cornerstone of electrocatalysis with further advances that bridge the gap with real systems.
- Serhiy Cherevko
- Ioannis Katsounaros
Discovering cryptic natural products by substrate manipulation
Cryptic halogenation reactions result in natural products with diverse structural motifs and bioactivities. However, these halogenated species are difficult to detect with current analytical methods because the final products are often not halogenated. An approach to identify products of cryptic halogenation using halide depletion has now been discovered, opening up space for more effective natural product discovery.
- Ludek Sehnal
- Libera Lo Presti
- Nadine Ziemert
Related Subjects
- Bioanalytical chemistry
- Circular dichroism
- Fluorescent probes
- Imaging studies
- Infrared spectroscopy
- Lab-on-a-chip
- Mass spectrometry
- Medical and clinical diagnostics
- Microfluidics
- NMR spectroscopy
- X-ray diffraction
Latest Research and Reviews
Diazobutanone-assisted isobaric labelling of phospholipids and sulfated glycolipids enables multiplexed quantitative lipidomics using tandem mass spectrometry
Lipidomics aims to uncover lipid functions in biological systems and disease. Quantifying lipids is challenging due to highly diverse chemical structures. Here a diazobutanone-assisted isobaric labelling method is developed that relies on diazobutanone and isobaric mass tags to target phosphate- and sulfate-containing lipids, enabling multiplexed lipidomic quantification in complex mixtures.
- Ting-Jia Gu
- Peng-Kai Liu
Nickel-organo compounds as potential enzyme precursors under simulated early Earth conditions
Nickel organic compounds play a crucial role in organic and bioinorganic chemistry, however, their potential role in prebiotic chemistry is not well understood. Here, the authors identify the formation of a nickel bis(dithiolene) from nickel sulfide under an acetylene-containing volcanic hydrothermal experimental environment and reveal its role in the transformation of acetylene to acetaldehyde.
- Philippe Diederich
- Christian Seitz
- Philippe Schmitt-Kopplin
A spectroscopic test suggests that fragment ion structure annotations in MS/MS libraries are frequently incorrect
Collision-induced dissociation tandem mass spectrometry offers to resolve molecular structures through library searches, however, for de novo identifications, one must often rely on in silico - generated spectra as reference. Here, the authors evaluate in silico generated MS n product ion structures by comparison with spectroscopically established structures and find that for 36 randomly selected MS/MS product ions, the vast majority of annotations in three major libraries are incorrect, primarily due to unaccounted for cyclization rearrangements.
- Lara van Tetering
- Sylvia Spies
Catalyst-free late-stage functionalization to assemble α-acyloxyenamide electrophiles for selectively profiling conserved lysine residues
Covalent probes are a powerful tool for investigating small molecule and protein interactions, however, the development of reactive warheads to form covalent probes remains underexplored. Here, the authors develop α -acyloxyenamide electrophiles to covalently bind to lysine residues, and selectively profile the conserved lysine residues of kinase in live cells.
- Yuanyuan Zhao
- Zhengqiu Li
Unveiling origins, composition, and appearance of ancient Islamic gold coins through elemental and smartphone-based colorimetric studies
- Roberto Sáez-Hernández
- María Josefa Luque
- M. Luisa Cervera
From MS/MS library implementation to molecular networks: Exploring oxylipin diversity with NEO-MSMS
- Anis Elloumi
- Lindsay Mas-Normand
- Jean-Marie Galano
News and Comment
Improving reproducibility of photocatalytic reactions—how to facilitate broad application of new methods.
Photocatalysis, as a powerful tool for synthetic transformations, has the potential to impact industrial applications. In this commentary, we discuss the challenges and requirements with respect to reproducing published photocatalytic reactions in the (life science) industry.
- Santiago Cañellas
- Manuel Nuño
- Elisabeth Speckmeier
Spectrum of the past
Thirty-four years ago, Curry and Rumelhart described a neural network-based approach to annotate tandem mass spectra. Their ideas foreshadowed several important developments in computational mass spectrometry over the past decade, but many of the challenges they discuss remain relevant today.
- Michael A. Skinnider
Engineering cyanine cyclizations for new fluorogenic probes
As the need for specific fluorescent probes that enable high sensitivity and super-resolution imaging experiments continues to grow, it is imperative to develop new, well-characterized methods to modulate the emission of fluorophores. Now, a general platform affords visible-to-NIR fluorogenic fluorophores by engineering a simple cyclization event into cyanine dyes.
- Quintashia D. Wilson
- Ellen M. Sletten
Lightly counting membrane proteins in native nanodiscs
To determine the physiologically relevant oligomeric form of membrane proteins is extremely challenging. Now an elegant method of counting the oligomers in membrane proteins in near-native states is presented, using photobleaching and nanodiscs formed directly from cellular membranes.
- Koushambi Mitra
- Yamuna Krishnan
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Themed collection Most popular 2021 analytical chemistry articles, 2021
DNA nanostructure-based nucleic acid probes: construction and biological applications
In recent years, DNA has been widely noted as a kind of material that can be used to construct building blocks for biosensing, in vivo imaging, drug development, and disease therapy because of its advantages of good biocompatibility and programmable properties.
Fluorescent small organic probes for biosensing
Small-molecule based fluorescent probes are increasingly important for the detection and imaging of biological signaling molecules due to their simplicity, high selectivity and sensitivity, whilst being non-invasive, and suitable for real-time analysis of living systems.
Near-infrared fluorescent molecular probes for imaging and diagnosis of nephro-urological diseases
Near-infrared fluorescent molecular probes with improved imaging depth and optimized biodistribution have been reviewed, showing great potential for diagnosis of nephro-urological diseases.
Activatable fluorescence sensors for in vivo bio-detection in the second near-infrared window
Fluorescence imaging in the second near-infrared (NIR-II, 1000–1700 nm) window has exhibited advantages of high optical resolution at deeper penetration ( ca. 5–20 mm) in bio-tissues owing to the reduced photon scattering and tissue autofluorescence.
Near-infrared fluorescent probes: a next-generation tool for protein-labeling applications
This minireview describes the development of NIR chemical probes for various protein-tag systems.
Structural and process controls of AIEgens for NIR-II theranostics
Structural and process controls of NIR-II AIEgens realize manipulating of radiative (R) and nonradiative (NR) decay for precise theranostics.
Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications
This review highlights the molecular design strategy of β-galactosidase-activatable probes from turn-on mode to ratiometric mode, from ACQ to AIE-active probes, from NIR-I to NIR-II imaging and dual-mode of chemo-fluoro-luminescence imaging.
CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) protein systems revolutionize genome engineering and advance analytical chemistry and diagnostic technology.
Phosphorescent metal complexes as theranostic anticancer agents: combining imaging and therapy in a single molecule
The recent development in phosphorescent iridium, ruthenium and rhenium complexes as theranostic anticancer agents is summarized.
The chronological evolution of small organic molecular fluorescent probes for thiols
The chronological evolution of small organic molecular fluorescent probes for thiols: from separation dependency analysis to cellular specific analysis, what's next?
HSA-Lys-161 covalent bound fluorescent dye for in vivo blood drug dynamic imaging and tumor mapping
HSA lysine-161 covalent bound quinoxaline–coumarin based fluorescent dye realized in situ blood drug concentration monitoring and tumor visualization.
Enzyme-activated near-infrared fluorogenic probe with high-efficiency intrahepatic targeting ability for visualization of drug-induced liver injury
We rationally designed a leucine aminopeptidase (LAP) activated fluorogenic probe hCy-CA-LAP with high hepatocyte-targeting ability for accurate and sensitive imaging of DILI.
Systematic investigation of the aza-Cope reaction for fluorescence imaging of formaldehyde in vitro and in vivo
Systematic investigation of various homoallylamines reveals N-p -methoxybenzyl homoallylamine as the optimal 2-aza-Cope reaction moiety for development of highly efficient formaldehyde fluorescent probes for in vitro and in vivo imaging.
Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe
Rather than tracking aging using the resting state, ROKS , an optical probe, was developed for evaluating the degree of aging dynamically by precisely monitoring the stress response of individuals under stress.
Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells
Acridonylalanine (Acd) is photostable, with a high quantum yield and long fluorescence lifetime in water. An evolved tRNA synthetase (RS) enables genetic incorporation of Acd in mammalian cells and its use in fluorescence lifetime imaging microscopy.
Turning waste into wealth: facile and green synthesis of carbon nanodots from pollutants and applications to bioimaging
The pollutant reactive red 2 was employed to synthesize fluorescent carbon nanodots allowing biological imaging in vitro and in vivo .
Activation of apoptosis by rationally constructing NIR amphiphilic AIEgens: surmounting the shackle of mitochondrial membrane potential for amplified tumor ablation
In this contribution, based on a “step-by-step” molecular design strategy, a novel NIR amphiphilic AIEgen TPA-S-TPP with a triplet lifetime of 11.43 μs and surmounting the shackle of MMP was successfully fabricated for amplified tumor ablation.
NIR-II cell endocytosis-activated fluorescent probes for in vivo high-contrast bioimaging diagnostics
A Cell Endocytosis-Activated Fluorescent (CEAF) probe triggered by disaggregation and protonation is designed for high contrast in vivo bioimaging and diagnostics in the second near-infrared window (1000–1700 nm).
Strategic engineering of alkyl spacer length for a pH-tolerant lysosome marker and dual organelle localization
A series naphthalimide-based fluorophores were designed by alkyl spacer length engineering to discover a pH-tolerant lysosomal marker. This approach also allows to probe lysosome-related organelles in C. elegans and communication between organelles.
Translating daily COVID-19 screening into a simple glucose test: a proof of concept study
COVID-19 glucose test: translating SARS-CoV-2 detection into a glucose test is achieved by incorporating target-responsive rolling circle amplification and a CRISPR-based collateral cleavage module with a portable glucose meter.
X-ray scattering reveals ion clustering of dilute chromium species in molten chloride medium
Ion clustering of dilute chromium species was unexpectedly revealed in a high-temperature molten chloride salt, challenging several long-held assumptions regarding specific ionic interactions and transport in molten ionic media.
Late-stage functionalisation of alkyne-modified phospha-xanthene dyes: lysosomal imaging using an off–on–off type of pH probe
A series of NIR-emissive phospha-xanthene dyes bearing an ethynyl group are reported. The late-stage functionalisation of the NIR dyes enables creation of multi-functionalised fluorescent probes that can be designed to target organelles of interest.
Synthesis of an AIEgen functionalized cucurbit[7]uril for subcellular bioimaging and synergistic photodynamic therapy and supramolecular chemotherapy
An AIEgen-functionalized cucurbit[7]uril was synthesized for the first time and spontaneously self-assembled into nanoaggregates in aqueous solutions and allowed subcellular imaging of the lysosome and photodynamic therapy and chemotherapy of cancer.
Rapid and ultrasensitive electrochemical detection of circulating tumor DNA by hybridization on the network of gold-coated magnetic nanoparticles
This study introduces a new electrochemical sensing strategy for the rapid detection of circulating tumor DNA (ctDNA) from whole blood in combination with a network of DNA-Au@MNPs with high sensitivity and excellent selectivity.
Design and synthesis of a ratiometric photoacoustic imaging probe activated by selenol for visual monitoring of pathological progression of autoimmune hepatitis
A ratiometric photoacoustic imaging probe activated by selenol was developed for visual monitoring of pathological progression of autoimmune hepatitis.
Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations
We report on a study that combines advanced fluorescence methods with molecular dynamics simulations to cover timescales from nanoseconds to milliseconds for a large protein, the chaperone Hsp90.
Single-molecule fluorescence detection of a tricyclic nucleoside analogue
Fluorescent nucleoside analogue ABN is readily detected at the single-molecule level and retains a quantum yield >50% in duplex DNA oligonucleotides.
Accelerated reactions of amines with carbon dioxide driven by superacid at the microdroplet interface
Microdroplets display distinctive interfacial chemistry, manifested as accelerated reactions relative to those observed for the same reagents in bulk.
Purely organic light-harvesting phosphorescence energy transfer by β-cyclodextrin pseudorotaxane for mitochondria targeted imaging
A new type of purely organic light-harvesting PET supramolecular assembly is constructed with efficient energy transfer and ultrahigh antenna effect. Moreover, the assembly could be used for mitochondria targeted imaging in A549 cancer cells.
All-in-one mitochondria-targeted NIR-II fluorophores for cancer therapy and imaging
Small-molecule subcellular organelle-targeting theranostic probes are crucial for early disease diagnosis and treatment.
About this collection
This specially curated collection pulls together some of the most popular articles from 2021 in the field of analytical chemistry. The collection presents some outstanding contributions to the field, ranging from fluorescent small organic probes for biosensing to electrochemical detection of circulating tumor DNA, and as with all Chemical Science articles – they are all completely free to access and read. We hope you enjoy browsing through this collection.
If a particular article has inspired you, do feel free to share on social media using the buttons on each article landing page and use our hashtag: #ChemSciMostPopular
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Journal of Analytical Chemistry
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Silver nanoparticles modified screen-printed electrochemical sensor: tool for control of pollutants of emerging concern in bee pollen.
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Development and Validation of a Stability Indicating HPLC Method for the Simultaneous Determination of Metformin, Hydrochlorothiazide and Acetylsalicylic Acid in an On-Demand Fixed-Dose Combination Dosage Form
- Jesús Alberto Afonso Urich
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Rapid Detection of Quinolone Antibiotics in Aquatic Products by Surface-Enhanced Raman Spectroscopy Combined with Thin Layer Chromatography
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A Novel Biological, Environmental, and Food Micro-Amount Slurry Samples Injection Technique for Simultaneous Quantification of Metals Using a Microwave Induced Plasma Optical Emission Spectrometry
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ASAP Articles are edited and published online ahead of issue. See all articles.
Bidimensional Dynamic Magnetic Levitation: Sequential Separation of Microplastics by Density and Size
- Xinpeng Ren
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- Fernando Maya*
FNICM: A New Methodology To Identify Core Metabolites Based on Significantly Perturbed Metabolic Subnetworks
- Gui-Zhong Xin*
Slow-Light-Enhanced Polarization Division Multiplexing Infrared Absorption Spectroscopy for On-Chip Wideband Multigas Detection in a 1D Photonic Crystal Waveguide
- Zihang Peng
- Yiding Wang
“Three-in-One” Multifunctional Hollow Nanocages with Colorimetric Photothermal Catalytic Activity for Enhancing Sensitivity in Biosensing
A rationally designed prodrug for the fluorogenic labeling of albumin and theranostic effects on drug-induced liver injury.
- Tianhong Wu
- Xiaolong Sun*
High-Performance Gel-Free and Label-Free Size Fractionation of Extracellular Vesicles with Two-Dimensional Electrophoresis in a Microfluidic Artificial Sieve
- Levent Yobas*
Efficient Discrimination of Hazardous Organophosphate Flame Retardants via Cataluminescence-Based Multidimensional Ratiometric Sensing
- Zhengjun Gong*
- Xiaoying Huang*
Hydrogen-Bonded Organic Cocrystal-Encapsulated Perovskite Nanocrystals as Coreactant-Free Electrochemiluminescent Luminophore for the Detection of Uranium
- Yuan-Jun Cai
- Jian-Ding Qiu*
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PyMIDA: A Graphical User Interface for Mass Isotopomer Distribution Analysis
- Naveed Ziari
- Marc K. Hellerstein *
Mass Spectrometry-Based Precise Identification of Citrullinated Histones via Limited Digestion and Biotin Derivative Tag Enrichment
- Lauren Fields
- Miriam A. Shelef
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- Lingjun Li *
Multiplatform High-Definition Ion Mobility Separations of the Largest Epimeric Peptides
- Hayden A. Thurman
- Gayani Wijegunawardena
- Francis Berthias
- David L. Williamson
- Ole N. Jensen
- Alexandre A. Shvartsburg *
Plasma-Droplet Fusion-Mass Spectrometry Reveals Sub-Millisecond Protein Unfolding Dynamics Induced by Reactive Oxygen Species
- Gongyu Li *
Classification of Molecular Binding Traces for Dynamic Single-Molecule Sensing
- Juntao Chen
- Yiyang Zhang
- Yuting Yang *
Single-Chain Conjugated Polymer Guests Confined inside Metal–Organic Frameworks (MOFs): Boosting the Detection and Degradation of a Sulfur Mustard Simulant
- Yongxian Guo
- Yifei Jiang
- Yanjun Gong
- Qiongzheng Hu *
Multiplex Aptamer-Based Fluorescence Assay Using Magnetism-Encoded Nanoparticles for Simultaneous Detection of Multiple Pathogenic Bacteria
- Yingjie Huang
- Jiang Huang
- Guangran Ma
- Yongjun Liu
- Hongliang Tan *
Enhanced Structure-Based Prediction of Chiral Stationary Phases for Chromatographic Enantioseparation from 3D Molecular Conformations
- Christopher J. Welch
- Patrick Piras
- Haixu Tang *
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- © 2023
Analytical Chemistry
Basic Techniques and Methods
- Priti Malhotra 0
Department of Chemistry, Daulat Ram College, University of Delhi, New Delhi, India
You can also search for this author in PubMed Google Scholar
Provides basic understanding of analytical chemistry
Covers analytical chemistry fundamentals and its analytical techniques
Discusses topics such as ultraviolet and visible spectral methods and infrared spectroscopy
8007 Accesses
1 Citations
3 Altmetric
- Table of contents
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Table of contents (21 chapters)
Front matter, statistical methods of analysis.
Priti Malhotra
- Spectroanalytical Techniques
- Ultraviolet and Visible Spectral Methods
Infrared Spectroscopy
Atomic absorption spectroscopy, atomic emission spectroscopy.
- Thermal Methods
Electroanalytical Methods
Conductometric methods, solvent extraction, chromatography, thin layer chromatography, column chromatography, gas chromatography, high performance liquid chromatography, ion exchange chromatography, exclusion chromatography.
- analytical chemistry
- chemical analysis
Book Title : Analytical Chemistry
Book Subtitle : Basic Techniques and Methods
Authors : Priti Malhotra
DOI : https://doi.org/10.1007/978-3-031-26757-4
Publisher : Springer Cham
eBook Packages : Chemistry and Materials Science , Chemistry and Material Science (R0)
Copyright Information : The Author(s) 2023
Hardcover ISBN : 978-3-031-26756-7 Published: 25 July 2023
Softcover ISBN : 978-3-031-26759-8 Due: 08 August 2024
eBook ISBN : 978-3-031-26757-4 Published: 24 July 2023
Edition Number : 1
Number of Pages : XV, 339
Number of Illustrations : 216 b/w illustrations, 2 illustrations in colour
Topics : Analytical Chemistry , Spectroscopy/Spectrometry , Crystallography and Scattering Methods
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